AIM: To describe the results of a Universal Newborn Hearing Screening program in a Low Risk Maternity Hospital. METHOD: This cross sectional and descriptive study was developed at the Gota de Leite Maternity Hospital, which is linked to the Municipal Health Department of Marilia. All neonates underwent hearing screening using transient evoked otoacoustic emissions (TEOAE) with AccuScreen (Madsen) equipment. The participants, who were screened in May 2013, were divided into 2 groups: Group I (GI) included 136 neonates without any risks of hearing loss and Group II (GII) included 16 neonates at a risk of hearing loss. RESULTS: A total of 152 hearing screens were performed over 1 month. The sample included 78 women and 74 men (mean age, 13 days). In GI, 132 (97.05%) neonates passed the screening test and in GII, 16 (100%) passed. The average time of the TEOAE was 20 seconds for both groups. The GII neonates presented the following risk indicators: family history of hearing loss, phototherapy, prematurity, low weight, and intensive care unit stay. CONCLUSION: In the neonate population studied, all of whom were screened within the recommended age range, the run time of the evaluation was brief, and there was a low rate of failure. No influence of risk factors on screening outcomes was observed. These findings reinforce the need for the implementation of universal newborn hearing screening.